Systems metabolic engineering of Escherichia coli for hyper-production of 5‑aminolevulinic acid.

ABSTRACT:

Background

5-Aminolevulinic acid (5-ALA) is a promising biostimulant, feed nutrient, and photodynamic drug with wide applications in modern agriculture and therapy. Although microbial production of 5-ALA has been improved realized by using metabolic engineering strategies during the past few years, there is still a gap between the present production level and the requirement of industrialization.

Results

In this study, pathway, protein, and cellular engineering strategies were systematically employed to construct an industrially competitive 5-ALA producing Escherichia coli. Pathways involved in precursor supply and product degradation were regulated by gene overexpression and synthetic sRNA-based repression to channel metabolic flux to 5-ALA biosynthesis. 5-ALA synthase was

SUBMITTER: Pu W 

PROVIDER: S-EPMC9951541 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature