Specific in situ immuno-imaging of pulmonary-resident memory lymphocytes in human lungs.

ABSTRACT:

Introduction

Pulmonary-resident memory T cells (T_RM) and B cells (B_RM) orchestrate protective immunity to reinfection with respiratory pathogens. Developing methods for the in situ detection of these populations would benefit both research and clinical settings.

Methods

To address this need, we developed a novel in situ immunolabelling approach combined with clinic-ready fibre-based optical endomicroscopy (OEM) to detect canonical markers of lymphocyte tissue residency in situ in human lungs undergoing ex vivo lung ventilation (EVLV).

Results

Initially, cells from human lung digests (confirmed to contain T_RM/B_RM populations using flow cytometry) were stained with CD69 and CD103/CD20 fluorescent an

SUBMITTER: Humphries DC 

PROVIDER: S-EPMC9951616 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature