Manipulation of diacylglycerol and ERK-mediated signaling differentially controls CD8⁺ T cell responses during chronic viral infection.

ABSTRACT:

Introduction

Activation of T cell receptor (TCR) signaling is critical for clonal expansion of CD8+ T cells. However, the effects of augmenting TCR signaling during chronic antigen exposure is less understood. Here, we investigated the role of diacylglycerol (DAG)-mediated signaling downstream of the TCR during chronic lymphocytic choriomeningitis virus clone 13 (LCMV CL13) infection by blocking DAG kinase zeta (DGKζ), a negative regulator of DAG.

Methods

We examined the activation, survival, expansion, and phenotype of virus-specific T cell in the acute and chronic phases of LCMV CL13-infected in mice after DGKζ blockade or selective activation of ERK.

Results

Upon LCMV CL13 infection, DGKζ deficiency promoted early short-lived effector cell (SLEC) differentiation of

SUBMITTER: Harabuchi S 

PROVIDER: S-EPMC9951774 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature