Experimental venous thrombus resolution is driven by IL-6 mediated monocyte actions.

ABSTRACT: Deep venous thrombosis and residual thrombus burden correlates with circulating IL-6 levels in humans. To investigate the cellular source and role of IL-6 in thrombus resolution, Wild type C57BL/6J (WT), and IL-6^-/- mice underwent induction of VT via inferior vena cava (IVC) stenosis or stasis. Vein wall (VW) and thrombus were analyzed by western blot, immunohistochemistry, and flow cytometry. Adoptive transfer of WT bone marrow derived monocytes was performed into IL6^-/- mice to assess for rescue. Cultured BMDMs from WT and IL-6^-/- mice underwent quantitative real time PCR and immunoblotting for fibrinolytic factors and matrix metalloproteinase activity. No differences in baseline coagulation function or platelet function were found between WT and IL-6

SUBMITTER: Obi AT 

PROVIDER: S-EPMC9951841 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature