Exploiting Kinetic Features of ORAC Assay for Evaluation of Radical Scavenging Capacity.

ABSTRACT: The analysis and interpretation of data retrieved from Oxygen Radical Absorbance Capacity (ORAC) assays represent a challenging task. ORAC indexes originate from different mathematical approaches often lacking correct elucidation of kinetic features concerning radical scavenging reactions by antioxidant compounds. In this work, the expression of ORAC values as area under fluorescein (FL) decay curves (AUC) and lag time are critically compared. This multi-parametric analysis showed the extension of radical scavenging reactions beyond the lag time period for caffeic acid, gallic acid, reduced glutathione and quercetin, extending their antioxidant protection of FL. Ethanol delayed the reaction of both FL and antioxidant compounds with free radical species generated from 2,2'-azobis(2-amidinop

SUBMITTER: Carvalho JRB 

PROVIDER: S-EPMC9951910 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature