No Chance to Survive: Mo-CBP₃-PepII Synthetic Peptide Acts on Cryptococcus neoformans by Multiple Mechanisms of Action.

ABSTRACT: Multidrug-resistant Cryptococcus neoformans is an encapsulated yeast causing a high mortality rate in immunocompromised patients. Recently, the synthetic peptide Mo-CBP₃-PepII emerged as a potent anticryptococcal molecule with an MIC₅₀ at low concentration. Here, the mechanisms of action of Mo-CBP₃-PepII were deeply analyzed to provide new information about how it led C. neoformans cells to death. Light and fluorescence microscopies, analysis of enzymatic activities, and proteomic analysis were employed to understand the effect of Mo-CBP₃-PepII on C. neoformans cells. Light and fluorescence microscopies revealed Mo-CBP₃-PepII induced the accumulation of anion superoxide and hydrogen pero

SUBMITTER: Aguiar TKB 

PROVIDER: S-EPMC9952340 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature