Dataset Information

The Duck RXRA Gene Promotes Adipogenesis and Correlates with Feed Efficiency.

ABSTRACT:

Background

The accumulation of fat in ducks is the main cause of low feed efficiency and metabolic diseases in ducks. Retinoic acid X receptor alpha (RXRA) is a member of the nuclear receptor superfamily involved in lipid, glucose, energy, and hormone metabolism. The effect of the RXRA gene on lipid metabolism in duck preadipocytes (DPACs) and the relationship between SNPs and the feed efficiency traits of ducks are unclear.

Methods

qRT-PCR and Western blotting analyses were used to detect changes in mRNA and protein in cells. Intracellular triglycerides (TGs) were detected using an ELISA kit. A general linear model analysis was used to determine the association between RXRA SNPs and feed efficiency.

Results

The duck RXRA gene was highly expressed on the fourth day of DPAC differentiation. The RXRA gene increased the content of fat and TG in DPACs and promoted the expression of cell differentiation genes; g.5,952,667 correlated with average daily feed intake (ADFI), residual feed intake (RFI), and feed conversion ratio (FCR).

Conclusions

Duck RXRA can accelerate fat accumulation, and the polymorphism of the RXRA gene is closely related to feed efficiency, which provides basic data for breeding high feed efficiency ducks.

SUBMITTER: Pan Z

PROVIDER: S-EPMC9952354 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature

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Publications

The Duck <i>RXRA</i> Gene Promotes Adipogenesis and Correlates with Feed Efficiency.

Pan Ziyi Z Li Xuewen X Wu Dongsheng D Chen Xingyong X Zhang Cheng C Jin Sihua S Geng Zhaoyu Z

Animals : an open access journal from MDPI 20230215 4

<h4>Background</h4>The accumulation of fat in ducks is the main cause of low feed efficiency and metabolic diseases in ducks. Retinoic acid X receptor alpha (<i>RXRA</i>) is a member of the nuclear receptor superfamily involved in lipid, glucose, energy, and hormone metabolism. The effect of the <i>RXRA</i> gene on lipid metabolism in duck preadipocytes (DPACs) and the relationship between SNPs and the feed efficiency traits of ducks are unclear.<h4>Methods</h4>qRT-PCR and Western blotting analy ...[more]

PMID: 36830469

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