Metabolomic Analysis of Multiple Biological Specimens (Feces, Serum, and Urine) by ¹H-NMR Spectroscopy from Dairy Cows with Clinical Mastitis.

ABSTRACT: Due to huge economic losses to the dairy industry worldwide, mastitis can be considered as one of the most common diseases in dairy cows. This work aimed to study this disease by comparing multiple biological specimens (feces, serum, and urine) from individuals with or without clinical mastitis. This was performed by a single analytical platform, namely ¹H-NMR, through a multi-matrix strategy. Thanks to the high reproducibility of ¹H-NMR, we could characterize 120 molecules across dairy cow feces, serum, and urine. Among them, 23 molecules were in common across the three biofluids. By integrating the results of multi-matrix metabolomics, several pathways pertaining to energy metabolism and amino acid metabolism appeared to be affected by clinical mastitis. The present

SUBMITTER: Zhu C 

PROVIDER: S-EPMC9952568 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature