Conversion of M1 Macrophages to Foam Cells: Transcriptome Differences Determined by Sex.

ABSTRACT:

Background

M1 macrophages involved in pro-inflammatory processes can be induced by low-density lipoproteins (LDL), giving rise to foam cells. In the atheroma plaque, it has been identified that males present more advanced lesions associated with infiltration. Therefore, our study aims to investigate sex-related changes in the transcriptome of M1 macrophages during the internalization process of LDL particles.

Methods

Peripheral blood mononuclear cells (PBMCs) from healthy male and female subjects were separated using Hystopaque, and monocytes were isolated from PBMCs using a positive selection of CD14+ cells. Cells were stimulated with LDL 10 µg/mL, and the transcriptional profile of M1 macrophages performed during LDL internalization was determined using a Clariom D platfor

SUBMITTER: Nambo-Venegas R 

PROVIDER: S-EPMC9953229 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature