Intracellular Sugar Transporters Facilitate Cellulase Synthesis in Trichoderma reesei Using Lactose.

ABSTRACT: Sugar transporters play an important role in the cellulase production of lignocellulose-degrading fungi. Nevertheless, the role and function of these transporters are still unclear. Here we first report intracellular sugar transporters assisting cellulase production in Trichoderma reesei (T. reesei) using lactose. The mRNA levels of sugar transporter genes mfs, gst, and lac1 were substantially upregulated in T. reesei cultivated on lactose, with the most abundant mRNA levels at 24 h as compared to glucose. Moreover, the individual deletion of these sugar transporters significantly inhibited cellulase production, solid cell growth, and sporulation of T. reesei, suggesting they play a supporting role in cellulase production when grown in lacto

SUBMITTER: Wang H 

PROVIDER: S-EPMC9953249 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature