Transcriptomic Analysis of Macrophage Polarization Protocols: Vitamin D₃ or IL-4 and IL-13 Do Not Polarize THP-1 Monocytes into Reliable M2 Macrophages.

ABSTRACT: Two main types of macrophages (Mφ) include inflammatory (M1) and anti-inflammatory (M2) macrophages. These cells can be obtained in vitro by polarization of monocytic cell lines using various stimuli. Since there is currently no consensus on the best method for the acquisition of reliable M1 and M2 macrophages from the THP-1 cell line, we decided to compare three different polarization protocols at the transcriptomic level. Whole transcriptomes of Mφ polarized according to the chosen protocols were analyzed using RNA-seq. Differential expression of genes and functional enrichment for gene ontology terms were assessed. Compared with other protocols, M1 macrophages polarized using PMA (61.3 ng/mL) and IFN-γ along with LPS had the highest expression of M1-associated regulatory genes and genes

SUBMITTER: Rynikova M 

PROVIDER: S-EPMC9953291 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature