Magnetic SERS Strip Based on 4-mercaptophenylboronic Acid-Modified Fe₃O₄@Au for Active Capture and Simultaneous Detection of Respiratory Bacteria.

ABSTRACT: The rapid diagnosis and detection of respiratory bacteria at the early stage can effectively control the epidemic spread and bacterial infection. Here, we designed a rapid, ultrasensitive, and quantitative lateral flow immunoassay (LFA) strip for simultaneous detection of respiratory bacteria S. aureus and S. pneumoniae. In this assay, the surface enhanced Raman scattering (SERS) tags were designed through combining magnetite Raman enhancement nanoparticle Fe₃O₄@Au/DTNB and recognition element 4-mercaptophenylboronic acid (4-MPBA). Further, 4-MPBA could capture multiple bacteria in a complex environmental solution. Based on the strategies, Fe₃O₄@Au/DTNB-mediated magnetic enrichment and 4-MPBA-mediated universal capture capabilities

SUBMITTER: Li J 

PROVIDER: S-EPMC9953780 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature