Modeling Blast Crisis Using Mutagenized Chronic Myeloid Leukemia-Derived Induced Pluripotent Stem Cells (iPSCs).

ABSTRACT:

Purpose

To model CML progression in vitro and generate a blast crisis (BC-CML) model in vitro in order to identify new targets.

Methods

Three different CML-derived iPSC lines were mutagenized with the alkylating agent ENU on a daily basis for 60 days. Cells were analyzed at D12 of hematopoietic differentiation for their phenotype, clonogenicity, and transcriptomic profile. Single-cell RNA-Seq analysis has been performed at three different time points during hematopoietic differentiation in ENU-treated and untreated cells.

Results

One of the CML-iPSCs, compared to its non-mutagenized counterpart, generated myeloid blasts after hematopoietic differentiation, exhibiting monoblastic patterns and expression of cMPO, CD45, CD34, CD33, and CD13. Single-cell transcriptomics r

SUBMITTER: Imeri J 

PROVIDER: S-EPMC9953961 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature