Fluholoscopy-Compact and Simple Platform Combining Fluorescence and Holographic Microscopy.

ABSTRACT: The combination of different imaging modalities into single imaging platforms has a strong potential in biomedical sciences as it permits the analysis of complementary properties of the target sample. Here, we report on an extremely simple, cost-effective, and compact microscope platform for achieving simultaneous fluorescence and quantitative phase imaging modes with the capability of working in a single snapshot. It is based on the use of a single illumination wavelength to both excite the sample's fluorescence and provide coherent illumination for phase imaging. After passing the microscope layout, the two imaging paths are separated using a bandpass filter, and the two imaging modes are simultaneously obtained using two digital cameras. We first present calibration and analysis of both

SUBMITTER: Alonso D 

PROVIDER: S-EPMC9954010 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature