Delineating Ultrafast Structural Dynamics of a Green-Red Fluorescent Protein for Calcium Sensing.

ABSTRACT: Fluorescent proteins (FPs) are indispensable tools for noninvasive bioimaging and sensing. Measuring the free cellular calcium (Ca²⁺) concentrations in vivo with genetically encodable FPs can be a relatively direct measure of neuronal activity due to the complex signaling role of these ions. REX-GECO1 is a recently developed red-green emission and excitation ratiometric FP-based biosensor that achieves a high dynamic range due to differences in the chromophore response to light excitation with and without calcium ions. Using steady-state electronic measurements (UV/Visible absorption and emission), along with time-resolved spectroscopic techniques including femtosecond transient absorption (fs-TA) and femtosecond stimulated Raman spectroscopy (FSRS), the potential energy

SUBMITTER: Krueger TD 

PROVIDER: S-EPMC9954042 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature