Validation of Rapid and Economic Colorimetric Nanoparticle Assay for SARS-CoV-2 RNA Detection in Saliva and Nasopharyngeal Swabs.

ABSTRACT: Even with the widespread uptake of vaccines, the SARS-CoV-2-induced COVID-19 pandemic continues to overwhelm many healthcare systems worldwide. Consequently, massive scale molecular diagnostic testing remains a key strategy to control the ongoing pandemic, and the need for instrument-free, economic and easy-to-use molecular diagnostic alternatives to PCR remains a goal of many healthcare providers, including WHO. We developed a test (Repvit) based on gold nanoparticles that can detect SARS-CoV-2 RNA directly from nasopharyngeal swab or saliva samples with a limit of detection (LOD) of 2.1 × 10⁵ copies mL^-1 by the naked eye (or 8 × 10⁴ copies mL^-1 by spectrophotometer) in less than 20 min, without the need for any instrumentation, and with a manuf

SUBMITTER: Armesto M 

PROVIDER: S-EPMC9954569 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature