An Optimized Workflow to Generate and Characterize iPSC-Derived Motor Neuron (MN) Spheroids.

ABSTRACT: A multitude of in vitro models based on induced pluripotent stem cell (iPSC)-derived motor neurons (MNs) have been developed to investigate the underlying causes of selective MN degeneration in motor neuron diseases (MNDs). For instance, spheroids are simple 3D models that have the potential to be generated in large numbers that can be used across different assays. In this study, we generated MN spheroids and developed a workflow to analyze them. To start, the morphological profiling of the spheroids was achieved by developing a pipeline to obtain measurements of their size and shape. Next, we confirmed the expression of different MN markers at the transcript and protein levels by qPCR and immunocytochemistry of tissue-cleared samples, respectively. Finally, we assessed the capacity of the

SUBMITTER: Castellanos-Montiel MJ 

PROVIDER: S-EPMC9954647 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature