MiR-218-5p/EGFR Signaling in Arsenic-Induced Carcinogenesis.

ABSTRACT:

Background

Arsenic is a well-known carcinogen inducing lung, skin, bladder, and liver cancer. Abnormal epidermal growth factor receptor (EGFR) expression is common in lung cancer; it is involved in cancer initiation, development, metastasis, and treatment resistance. However, the underlying mechanism for arsenic-inducing EGFR upregulation remains unclear.

Methods

RT-PCR and immunoblotting assays were used to detect the levels of miR-218-5p and EGFR expression. The Luciferase assay was used to test the transcriptional activity of EGFR mediated by miR-218-5p. Cell proliferation, colony formation, wound healing, migration assays, tube formation assays, and tumor growth assays were used to study the function of miR-218-5p/EGFR signaling.

Results

EGFR and miR-218-5p were d

SUBMITTER: Islam R 

PROVIDER: S-EPMC9954652 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature