Development and Optimization of Label-Free Quantitative Proteomics under Different Crossing Periods of Bottle Gourd.

ABSTRACT: Bottle gourd, a common vegetable in the human diet, has been valued for its medicinal and energetic properties. In this experiment, the time-resolved analysis of the changes in the proteins' electrophoretic patterning of the seed development at different crossing periods was studied in bottle gourd using label-free quantitative proteomics. Hybrid HBGH-35 had the highest observed protein levels at the 4th week of the crossing period (F₄) compared to the parental lines, viz. G-2 (M) and Pusa Naveen (F). The crossing period is significantly correlated with grain filling and reserve accumulation. The observed protein expression profile after storage was related to seed maturation and grain filling in bottle gourds. A total of 2517 proteins were identified in differentially treated b

SUBMITTER: Malik A 

PROVIDER: S-EPMC9955185 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature