Inositol pyrophosphate profiling reveals regulatory roles of IP6K2-dependent enhanced IP₇ metabolism in the enteric nervous system.

ABSTRACT: Inositol pyrophosphates regulate diverse physiological processes; to better understand their functional roles, assessing their tissue-specific distribution is important. Here, we profiled inositol pyrophosphate levels in mammalian organs using an originally designed liquid chromatography-mass spectrometry (LC-MS) protocol and discovered that the gastrointestinal tract (GIT) contained the highest levels of diphosphoinositol pentakisphosphate (IP₇) and its precursor inositol hexakisphosphate (IP₆). Although their absolute levels in the GIT are diet dependent, elevated IP₇ metabolism still exists under dietary regimens devoid of exogenous IP₇. Of the major GIT cells, enteric neurons selectively express the IP₇-synthesizing enzyme IP6K2. W

SUBMITTER: Ito M 

PROVIDER: S-EPMC9957762 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature