Multi-level Force-dependent Allosteric Enhancement of αE-catenin Binding to F-actin by Vinculin.

ABSTRACT: Classical cadherins are transmembrane proteins whose extracellular domains link neighboring cells, and whose intracellular domains connect to the actin cytoskeleton via β-catenin and α-catenin. The cadherin-catenin complex transmits forces that drive tissue morphogenesis and wound healing. In addition, tension-dependent changes in αE-catenin conformation enables it to recruit the actin-binding protein vinculin to cell-cell junctions, which contributes to junctional strengthening. How and whether multiple cadherin-complexes cooperate to reinforce cell-cell junctions in response to load remains poorly understood. Here, we used single-molecule optical trap measurements to examine how multiple cadherin-catenin complexes interact with F-actin under load, and how this interaction is influenced b

SUBMITTER: Bax NA 

PROVIDER: S-EPMC9957948 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature