Molecular imaging of liver inflammation using an anti-VCAM-1 nanobody.

ABSTRACT: To date, a biopsy is mandatory to evaluate parenchymal inflammation in the liver. Here, we evaluated whether molecular imaging of vascular cell adhesion molecule-1 (VCAM-1) could be used as an alternative non-invasive tool to detect liver inflammation in the setting of chronic liver disease. To do so, we radiolabeled anti-VCAM-1 nanobody (^99mTc-cAbVCAM1-5) and used single-photon emission computed tomography (SPECT) to quantify liver uptake in preclinical models of non-alcoholic fatty liver disease (NAFLD) with various degree of liver inflammation: wild-type mice fed a normal or high-fat diet (HFD), FOZ fed a HFD and C57BL6/J fed a choline-deficient or -supplemented HFD. ^99mTc-cAbVCAM1-5 uptake strongly correlates with liver histological inflammatory score and with mol

SUBMITTER: Nachit M 

PROVIDER: S-EPMC9957989 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature