Nanowarming and ice-free cryopreservation of large sized, intact porcine articular cartilage.

ABSTRACT: Successful organ or tissue long-term preservation would revolutionize biomedicine. Cartilage cryopreservation enables prolonged shelf life of articular cartilage, posing the prospect to broaden the implementation of promising osteochondral allograft (OCA) transplantation for cartilage repair. However, cryopreserved large sized cartilage cannot be successfully warmed with the conventional convection warming approach due to its limited warming rate, blocking its clinical potential. Here, we develope a nanowarming and ice-free cryopreservation method for large sized, intact articular cartilage preservation. Our method achieves a heating rate of 76.8 °C min^-1, over one order of magnitude higher than convection warming (4.8 °C min^-1). Using systematic cell and tissue level

SUBMITTER: Chen P 

PROVIDER: S-EPMC9958003 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature