Absolute quantification of cellular levels of photosynthesis-related proteins in Synechocystis sp. PCC 6803.

ABSTRACT: Quantifying cellular components is a basic and important step for understanding how a cell works, how it responds to environmental changes, and for re-engineering cells to produce valuable metabolites and increased biomass. We quantified proteins in the model cyanobacterium Synechocystis sp. PCC 6803 given the general importance of cyanobacteria for global photosynthesis, for synthetic biology and biotechnology research, and their ancestral relationship to the chloroplasts of plants. Four mass spectrometry methods were used to quantify cellular components involved in the biosynthesis of chlorophyll, carotenoid and bilin pigments, membrane assembly, the light reactions of photosynthesis, fixation of carbon dioxide and nitrogen, and hydrogen and sulfur metabolism. Components of biosynthetic

SUBMITTER: Jackson PJ 

PROVIDER: S-EPMC9958174 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature