Proteomic Analysis of Ferrochelatase Interactome in Erythroid and Non-Erythroid Cells.

ABSTRACT: Heme is an essential cofactor for multiple cellular processes in most organisms. In developing erythroid cells, the demand for heme synthesis is high, but is significantly lower in non-erythroid cells. While the biosynthesis of heme in metazoans is well understood, the tissue-specific regulation of the pathway is less explored. To better understand this, we analyzed the mitochondrial heme metabolon in erythroid and non-erythroid cell lines from the perspective of ferrochelatase (FECH), the terminal enzyme in the heme biosynthetic pathway. Affinity purification of FLAG-tagged-FECH, together with mass spectrometric analysis, was carried out to identify putative protein partners in human and murine cell lines. Proteins involved in the heme biosynthetic process and mitochondrial organization w

SUBMITTER: Obi CD 

PROVIDER: S-EPMC9958551 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature