Development of a Qualitative Test to Detect the Presence of Organophosphate Pesticides on Fruits and Vegetables.

ABSTRACT:

Background

In recent decades, the use of pesticides in agriculture has increased at a fast pace, highlighting safety problems for the environment and human health, which in turn has made it necessary to develop new detection and decontamination systems for pesticides.

Methods

A new qualitative test capable of detecting the presence of pesticides on fruits and vegetables by using thermostable enzymes was discovered, and the test was carried out on apples and aubergines. The contaminating pesticides were extracted from fruits with acetonitrile and analyzed with a biosensor system based on the thermostable esterase EST2 immobilized on a nitrocellulose filter. This enzyme is irreversibly inhibited mainly in the presence of organophosphates pesticides. Therefore, by observing est

SUBMITTER: De Luca V 

PROVIDER: S-EPMC9958579 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature