Exploration of the Nuclear Proteomes in the Ciliate Oxytricha trifallax.

ABSTRACT: Nuclear dimorphism is a fundamental feature of ciliated protozoa, which have separate somatic and germline genomes in two distinct organelles within a single cell. The transcriptionally active somatic genome, contained within the physically larger macronucleus, is both structurally and functionally different from the silent germline genome housed in the smaller micronucleus. This difference in genome architecture is particularly exaggerated in Oxytricha trifallax, in which the somatic genome comprises tens of thousands of gene-sized nanochromosomes maintained at a high and variable ploidy, while the germline has a diploid set of megabase-scale chromosomes. To examine the compositional differences between the nuclear structures housing the genomes, we performed a proteomic survey of

SUBMITTER: Lu MW 

PROVIDER: S-EPMC9958989 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature