A versatile multimodal chromatography strategy to rapidly purify protein nanostructures assembled in cell lysates.

ABSTRACT:

Background

Protein nanostructures produced through the self-assembly of individual subunits are attractive scaffolds to attach and position functional molecules for applications in biomaterials, metabolic engineering, tissue engineering, and a plethora of nanomaterials. However, the assembly of multicomponent protein nanomaterials is generally a laborious process that requires each protein component to be separately expressed and purified prior to assembly. Moreover, excess components not incorporated into the final assembly must be removed from the solution and thereby necessitate additional processing steps.

Results

We developed an efficient approach to purify functionalized protein nanostructures directly from bacterial lysates through a type of multimodal chromatography

SUBMITTER: Winter DL 

PROVIDER: S-EPMC9960191 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature