Structural and DNA end resection study of the bacterial NurA-HerA complex.

ABSTRACT:

Background

The nuclease NurA and the ATPase/translocase HerA play a vital role in repair of double-strand breaks (DSB) during the homologous recombination in archaea. A NurA-HerA complex is known to mediate DSB DNA end resection, leading to formation of a free 3' end used to search for the homologous sequence. Despite the structures of individual archaeal types of NurA and HerA having been reported, there is limited information regarding the molecular mechanisms underlying this process. Some bacteria also possess homologs of NurA and HerA; however, the bacterial type of this complex, as well as the detailed mechanisms underlying the joining of NurA-HerA in DSB DNA end resection, remains unclear.

Results

We report for the first time the crystal structures of Deinococcus radio

SUBMITTER: Yang J 

PROVIDER: S-EPMC9960219 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature