Efficient prioritization of CRISPR screen hits by accounting for targeting efficiency of guide RNA.

ABSTRACT:

Background

CRISPR-based screens are revolutionizing drug discovery as tools to identify genes whose ablation induces a phenotype of interest. For instance, CRISPR-Cas9 screening has been successfully used to identify novel therapeutic targets in cancer where disruption of genes leads to decreased viability of malignant cells. However, low-activity guide RNAs may give rise to variable changes in phenotype, preventing easy identification of hits and leading to false negative results. Therefore, correcting the effects of bias due to differences in guide RNA efficiency in CRISPR screening data can improve the efficiency of prioritizing hits for further validation. Here, we developed an approach to identify hits from negative CRISPR screens by correcting the fold changes (FC) in gRNA fr

SUBMITTER: Park BS 

PROVIDER: S-EPMC9960226 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature