Quantifying organellar ultrastructure in cryo-electron tomography using a surface morphometrics pipeline.

ABSTRACT: Cellular cryo-electron tomography (cryo-ET) enables three-dimensional reconstructions of organelles in their native cellular environment at subnanometer resolution. However, quantifying ultrastructural features of pleomorphic organelles in three dimensions is challenging, as is defining the significance of observed changes induced by specific cellular perturbations. To address this challenge, we established a semiautomated workflow to segment organellar membranes and reconstruct their underlying surface geometry in cryo-ET. To complement this workflow, we developed an open-source suite of ultrastructural quantifications, integrated into a single pipeline called the surface morphometrics pipeline. This pipeline enables rapid modeling of complex membrane structures and allows detailed mappin

SUBMITTER: Barad BA 

PROVIDER: S-EPMC9960335 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature