Asbestos Fibers Enhance the TMEM16A Channel Activity in Xenopus Oocytes.

ABSTRACT:

Background

The interaction of asbestos fibers with target cell membranes is still poorly investigated. Here, we detected and characterized an enhancement of chloride conductance in Xenopus oocyte cell membranes induced by exposure to crocidolite (Croc) asbestos fibers.

Methods

A two-microelectrode voltage clamp technique was used to test the effect of Croc fiber suspensions on outward chloride currents evoked by step membrane depolarization. Calcium imaging experiments were also performed to investigate the variation of 'resting' oocyte [Ca²⁺]_i following asbestos exposure.

Results

The increase in chloride current after asbestos treatment, was sensitive to [Ca²⁺]_e, and to specific blockers of TMEM16A Ca²⁺-ac

SUBMITTER: Bernareggi A 

PROVIDER: S-EPMC9960392 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature