Detection and characterization of constitutive replication origins defined by DNA polymerase epsilon.

ABSTRACT:

Background

Despite the process of DNA replication being mechanistically highly conserved, the location of origins of replication (ORI) may vary from one tissue to the next, or between rounds of replication in eukaryotes, suggesting flexibility in the choice of locations to initiate replication. Lists of human ORI therefore vary widely in number and location, and there are currently no methods available to compare them. Here, we propose a method of detection of ORI based on somatic mutation patterns generated by the mutator phenotype of damaged DNA polymerase epsilon (POLE).

Results

We report the genome-wide localization of constitutive ORI in POLE-mutated human tumors using whole genome sequencing data. Mutations accumulated after many rounds of replication of unsynchronized

SUBMITTER: Jaksik R 

PROVIDER: S-EPMC9960419 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature