Transcriptomic changes in single yeast cells under various stress conditions.

ABSTRACT:

Background

The stress response of Saccharomyces cerevisiae has been extensively studied in the past decade. However, with the advent of recent technology in single-cell transcriptome profiling, there is a new opportunity to expand and further understanding of the yeast stress response with greater resolution on a system level. To understand transcriptomic changes in baker's yeast S. cerevisiae cells under stress conditions, we sequenced 117 yeast cells under three stress treatments (hypotonic condition, glucose starvation and amino acid starvation) using a full-length single-cell RNA-Seq method.

Results

We found that though single cells from the same treatment showed varying degrees of uniformity, technical noise and batch effects can confound results significantly. However,

SUBMITTER: Su Y 

PROVIDER: S-EPMC9960639 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature