Trioleyl Pyridinium, a Cationic Transfection Agent for the Lipofection of Therapeutic Oligonucleotides into Mammalian Cells.

ABSTRACT:

Background

One of the most significant limitations that therapeutic oligonucleotides present is the development of specific and efficient delivery vectors for the internalization of nucleic acids into cells. Therefore, there is a need for the development of new transfection agents that ensure a proper and efficient delivery into mammalian cells.

Methods

We describe the synthesis of 1,3,5-tris[(4-oelyl-1-pyridinio)methyl]benzene tribromide (TROPY) and proceeded to the validation of its binding capacity toward oligonucleotides, the internalization of DNA into the cells, the effect on cell viability, apoptosis, and its capability to transfect plasmid DNA.

Results

The synthesis and chemical characterization of TROPY, which can bind DNA and transfect oligonucleotides into

SUBMITTER: Delgado A 

PROVIDER: S-EPMC9960667 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature