Suppression of the HOS1 Gene Affects the Level of ROS Depending on Light and Cold.

ABSTRACT: The E3 ubiquitin-protein ligase HOS1 is an important integrator of temperature information and developmental processes. HOS1 is a negative regulator of plant cold tolerance, and silencing HOS1 leads to increased cold tolerance. In the present work, we studied ROS levels in hos1^Cas9Arabidopsis thaliana plants, in which the HOS1 gene was silenced by disruption of the open reading frame via CRISPR/Cas9 technology. Confocal imaging of intracellular reactive oxygen species (ROS) showed that the hos1 mutation moderately increased levels of ROS under both low and high light (HL) conditions, but wild-type (WT) and hos1^Cas9 plants exhibited similar ROS levels in the dark. Visualization of single cells did not reveal differences in the

SUBMITTER: Gorpenchenko TY 

PROVIDER: S-EPMC9960889 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature