Blood Cell Separation Using Polypropylene-Based Microfluidic Devices Based on Deterministic Lateral Displacement.

ABSTRACT: Mammalian blood cell separation methods contribute to improving the diagnosis and treatment of animal and human diseases. Microfluidic deterministic lateral displacement (DLD) devices can sort cells based on their particle diameter. We developed microfluidic DLD devices with poly(propylene)-based resin and used them to separate bovine and human red blood cells (RBCs) and white blood cells (WBCs) without electric devices. To determine the critical cut-off diameter (D_c) of these devices, we used immunobeads with a diameter of 1-20 μm. The D_c values of the microfluidic DLD devices for the immunobeads in the experiments were similar to the calculated D_c values (8-10 μm). Results from bovine blood cell separation experiments suggest that lymphocytes and neutroph

SUBMITTER: Matsuura K 

PROVIDER: S-EPMC9960998 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature