Activation of Endothelial Large Conductance Potassium Channels Protects against TNF-α-Induced Inflammation.

ABSTRACT: Elevated TNF-α levels in serum and broncho-alveolar lavage fluid of acute lung injury patients correlate with mortality rates. We hypothesized that pharmacological plasma membrane potential (Em) hyperpolarization protects against TNF-α-induced CCL-2 and IL-6 secretion from human pulmonary endothelial cells through inhibition of inflammatory Ca²⁺-dependent MAPK pathways. Since the role of Ca²⁺ influx in TNF-α-mediated inflammation remains poorly understood, we explored the role of L-type voltage-gated Ca²⁺ (Ca_V) channels in TNF-α-induced CCL-2 and IL-6 secretion from human pulmonary endothelial cells. The Ca_V channel blocker, Nifedipine, decreased both CCL-2 and IL-6 secretion, suggesting that a fraction of Ca_V channels is op

SUBMITTER: Zyrianova T 

PROVIDER: S-EPMC9961193 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature