Mammalian Oocyte Analysis by MALDI MSI with Wet-Interface Matrix Deposition Technique.

ABSTRACT: Oocytes are a special kind of biological material. Here, the individual variability of a single cell is important. It means that the opportunity to obtain information about the lipid content from the analysis of a single cell is significant. In our study, we present a method for lipid analysis based on the MALDI-based mass spectrometry imaging (MSI) approach. Our attention was paid to the sample preparation optimization with the aid of a wet-interface matrix deposition system (matrix spraying). Technical considerations of the sample preparation process, such as the number of matrix layers and the position of the spraying nozzle during the matrix deposition, are presented in the article. Additionally, we checked if changing the 2,5-dihydroxybenzoic acid (DHB) and 9-Aminoacridine (9AA) matri

SUBMITTER: Bodzon-Kulakowska A 

PROVIDER: S-EPMC9961235 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature