High-Molecular-Weight Plasmids Carrying Carbapenemase Genes bla_NDM-1, bla_KPC-2, and bla_OXA-48 Coexisting in Clinical Klebsiella pneumoniae Strains of ST39.

ABSTRACT:

Background

Klebsiella pneumoniae, a member of the ESKAPE group of bacterial pathogens, has developed multi-antimicrobial resistance (AMR), including resistance to carbapenems, which has increased alarmingly due to the acquisition of carbapenemase genes located on specific plasmids.

Methods

Four clinical K. pneumoniae isolates were collected from four patients of a neuro-intensive care unit in Moscow, Russia, during the point prevalence survey. The AMR phenotype was estimated using the Vitec-2 instrument, and whole genome sequencing (WGS) was done using Illumina and Nanopore technologies.

Results

All strains were resistant to beta-lactams, nitrofurans, fluoroquinolones, sulfonamides, aminoglycosides, and tetracyclines. WGS analysis revealed that all strai

SUBMITTER: Kuzina ES 

PROVIDER: S-EPMC9961262 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature