Pseudomonas Phage ZCPS1 Endolysin as a Potential Therapeutic Agent.

ABSTRACT: The challenge of antibiotic resistance has gained much attention in recent years due to the rapid emergence of resistant bacteria infecting humans and risking industries. Thus, alternatives to antibiotics are being actively searched for. In this regard, bacteriophages and their enzymes, such as endolysins, are a very attractive alternative. Endolysins are the lytic enzymes, which are produced during the late phase of the lytic bacteriophage replication cycle to target the bacterial cell walls for progeny release. Here, we cloned, expressed, and purified LysZC1 endolysin from Pseudomonas phage ZCPS1. The structural alignment, molecular dynamic simulation, and CD studies suggested LysZC1 to be majorly helical, which is highly similar to various phage-encoded lysozymes with glycoside h

SUBMITTER: Abdelrahman F 

PROVIDER: S-EPMC9961711 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature