Arachidonic Acid Added during the Differentiation Phase of 3T3-L1 Cells Exerts Anti-Adipogenic Effect by Reducing the Effects of Pro-Adipogenic Prostaglandins.

ABSTRACT: A linoleic acid (LA) metabolite arachidonic acid (AA) added to 3T3-L1 cells is reported to suppress adipogenesis. The purpose of the present study aimed to clarify the effects of AA added during the differentiation phase, including adipogenesis, the types of prostaglandins (PG)s produced, and the crosstalk between AA and the PGs produced. Adipogenesis was inhibited by AA added, while LA did not. When AA was added, increased PGE₂ and PGF_2α production, unchanged Δ¹²-PGJ₂ production, and reduced PGI₂ production were observed. Since the decreased PGI₂ production was reflected in decreased CCAAT/enhancer-binding protein-β (C/EBPβ) and C/EBPδ expression, we expected that the coexistence of PGI₂ with AA would suppress th

SUBMITTER: Nartey MNN 

PROVIDER: S-EPMC9962328 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature