Modified Linear Peptides Effectively Silence STAT-3 in Breast Cancer and Ovarian Cancer Cell Lines.

ABSTRACT: RNA interference (RNAi) has drawn enormous attention as a powerful tool because of its capability to interfere with mRNA and protein production. However, designing a safe and efficient delivery system in RNAi therapeutics remains challenging. Herein, we have designed and synthesized several linear peptides containing tryptophan (W) and arginine (R) residues separated by the β-alanine (βA) spacer and attached to a lipophilic fatty acyl chain, cholesterol, or PEG. The peptide backbone sequences were: Ac-C-βA-βA-W₄-βA-βA-R₄-CO-NH₂ and Ac-K-βA-βA-W₄-βA-βA-R₄-CO-NH₂, with only a difference in N-terminal amino acid. The cysteine side chain in the first sequence was used for the conjugation with PEG2000 and PEG550. Alternati

SUBMITTER: Mandal D 

PROVIDER: S-EPMC9962452 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature