Quantitative analysis of sterol-modulated monomer-dimer equilibrium of the β₁-adrenergic receptor by DEER spectroscopy.

ABSTRACT: G protein-coupled receptors (GPCR) activate numerous intracellular signaling pathways. The oligomerization properties of GPCRs, and hence their cellular functions, may be modulated by various components within the cell membrane (such as the presence of cholesterol). Modulation may occur directly via specific interaction with the GPCR or indirectly by affecting the physical properties of the membrane. Here, we use pulsed Q-band double electron-electron resonance (DEER) spectroscopy to probe distances between R1 nitroxide spin labels attached to Cys163 and Cys344 of the β₁-adrenergic receptor (β₁AR) in n-dodecyl-β-D-maltoside micelles upon titration with two soluble cholesterol analogs, cholesteryl hemisuccinate (CHS) and sodium cholate. The former, like choleste

SUBMITTER: Kubatova N 

PROVIDER: S-EPMC9963004 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature