Using LC-MS/MS to Determine Salivary Steroid Reference Intervals in a European Older Adult Population.

ABSTRACT: A number of steroids, including glucocorticoids and sex hormones, have been associated with neurodegenerative and cardiovascular conditions common in aging populations. The application of liquid chromatography tandem mass spectrometry (LC-MS/MS) steroid analysis offers an opportunity to conduct simultaneous multiplex steroid analysis within a given sample. In this paper, we describe the application of an LC-MS/MS steroid analysis method for the assessment of reference ranges of steroids in human saliva samples (200 µL) collected from older adults (age 50 years and above) enrolled in a European cohort investigating the risk for Alzheimer's dementia. Saliva samples were prepared using supported liquid extraction (SLE) along with a calibration curve and analysed using a Waters I-Class UPLC (U

SUBMITTER: Gregory S 

PROVIDER: S-EPMC9963097 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature