Molecular Cloning and Functional Characterization of Galectin-1 in Yellow Drum (Nibea albiflora).

ABSTRACT: Galectins are proteins that are involved in the innate immune response against pathogenic microorganisms. In the present study, the gene expression pattern of galectin-1 (named as NaGal-1) and its function in mediating the defense response to bacterial attack were investigated. The tertiary structure of NaGal-1 protein consists of homodimers and each subunit has one carbohydrate recognition domain. Quantitative RT-PCR analysis indicated that NaGal-1 was ubiquitously distributed in all the detected tissues and highly expressed in the swim-bladder of Nibea albiflora, and its expression could be upregulated by the pathogenic Vibrio harveyi attack in the brain. Expression of NaGal-1 protein in HEK 293T cells was distributed in the cytoplasm as w

SUBMITTER: Wu B 

PROVIDER: S-EPMC9963236 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature