Tubuloid culture enables long-term expansion of functional human kidney tubule epithelium from iPSC-derived organoids.

ABSTRACT: Kidney organoids generated from induced pluripotent stem cells (iPSC) have proven valuable for studies of kidney development, disease, and therapeutic screening. However, specific applications have been hampered by limited expansion capacity, immaturity, off-target cells, and inability to access the apical side. Here, we apply recently developed tubuloid protocols to purify and propagate kidney epithelium from d7+18 (post nephrogenesis) iPSC-derived organoids. The resulting 'iPSC organoid-derived (iPSCod)' tubuloids can be exponentially expanded for at least 2.5 mo, while retaining expression of important tubular transporters and segment-specific markers. This approach allows for selective propagation of the mature tubular epithelium, as immature cells, stroma, and undesirable off-target c

SUBMITTER: Yousef Yengej FA 

PROVIDER: S-EPMC9963523 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature