Allosteric mechanism of transcription inhibition by NusG-dependent pausing of RNA polymerase.

ABSTRACT: NusG is a transcription elongation factor that stimulates transcription pausing in Gram+ bacteria including B. subtilis by sequence-specific interaction with a conserved pause-inducing _-11TTNTTT_-6 motif found in the non-template DNA (ntDNA) strand within the transcription bubble. To reveal the structural basis of NusG-dependent pausing, we determined a cryo-EM structure of a paused transcription complex (PTC) containing RNA polymerase (RNAP), NusG, and the TTNTTT motif in the ntDNA strand. The interaction of NusG with the ntDNA strand rearranges the transcription bubble by positioning three consecutive T residues in a cleft between NusG and the β-lobe domain of RNAP. We revealed that the RNAP swivel module rotation (swiveling), which widens (swiveled

SUBMITTER: Vishwakarma RK 

PROVIDER: S-EPMC9963633 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature