High-throughput droplet-based analysis of influenza A virus genetic reassortment by single-virus RNA sequencing.

ABSTRACT: The segmented RNA genome of influenza A viruses (IAVs) enables viral evolution through genetic reassortment after multiple IAVs coinfect the same cell, leading to viruses harboring combinations of eight genomic segments from distinct parental viruses. Existing data indicate that reassortant genotypes are not equiprobable; however, the low throughput of available virology techniques does not allow quantitative analysis. Here, we have developed a high-throughput single-cell droplet microfluidic system allowing encapsulation of IAV-infected cells, each cell being infected by a single progeny virion resulting from a coinfection process. Customized barcoded primers for targeted viral RNA sequencing enabled the analysis of 18,422 viral genotypes resulting from coinfection with two circulating hu

SUBMITTER: Chen KY 

PROVIDER: S-EPMC9963642 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature