Cloning of an Albino Mutation of Arabidopsis thaliana Using Mapping-by-Sequencing.

ABSTRACT: We report the molecular characterization of an ethyl methanesulfonate (EMS)-induced mutation that causes albinism and lethality at the seedling stage in Arabidopsis thaliana. We identified the mutation using a mapping-by-sequencing approach that uses Fisher's exact tests to detect changes in allele frequencies among the seedlings of an F₂ mapping population, which had been pooled according to their phenotypes (wild-type or mutant). After purifying genomic DNA from the plants of both pools, the two samples were sequenced using the Illumina HiSeq 2500 next-generation sequencing platform. The bioinformatic analysis allowed us to identify a point mutation that damages a conserved residue at the acceptor site of an intron of the At2g04030 gene, which encodes the chloroplast-lo

SUBMITTER: Rodriguez-Alcocer E 

PROVIDER: S-EPMC9964061 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature